Biocompatibility test with Artemia salina for five materials for endodontic use

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Published: Jun 30, 2022
DOI: https://doi.org/10.21676/2389783X.5360
Keywords:
Artemia salina, Biocompatibility, Cytotoxicity, Protocols
Biocompatibility test with Artemia salina for five materials for endodontic use

Main Article Content

Leidy Johanna Torres-Avirama
Universidad del Valle
Daniela Alexandra Realpe-Urbano
Universidad del Valle
Tania Lizeth Guevara-Valencia
Universidad del Valle
Gilbert Alfonso Morales
Universidad del Valle
Ingrid Ximena Zamora-Córdoba
Universidad del Valle
Carlos Humberto Valencia-Llano
Universidad del Valle

Abstract

Introduction: Biocompatibility is a decisive factor in the success of endodontic therapy. Objective: to assess the cytotoxicity of five endodontic materials using the Artemia salina test. Method: an in vitro experimental study was designed, using Artemia salina, to evaluate the biocompatibility of Grossman, CaOH, AH-Plus, MTA, and TheraCal-LC cements, with observations at 24 and 48 hours. Results: The results indicate that Grossman cement presents 100% toxicity at 24 hours, while the other materials show values lower than 30%, reflecting a biocompatible behavior; however, at 48 hours, CaOH and TheraCal-LC present toxicity higher than 30%, while AH-Plus and MTA continue with values lower than 30%. The data were analyzed with a Generalized Linear Model with binomial error distribution, a deviancy analysis, and Fisher's multiple comparison test with Bonferroni correction. An independent analysis was performed for 24 and 48 hours, under a significance level α=5%; significant differences were found at 48 hours between TheraCal-LC and AH-Plus, and MTA. Conclusion: It is concluded that Grossman cement presented a high degree of toxicity, while AH-Plus and MTA showed the highest biocompatibility.

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How to Cite
Torres-Avirama, . L. J., Realpe-Urbano, D. A., Guevara-Valencia, T. L., Morales, G. A., Zamora-Córdoba, I. X., & Valencia-Llano, C. H. (2022). Biocompatibility test with Artemia salina for five materials for endodontic use. Duazary, 20(2), 105–114. https://doi.org/10.21676/2389783X.5360
Issue
Vol. 20 No. 2 (2023): (April - June)
Section
Articles of scientific and technological research
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