Antifungal effect of Crescentia cujete fruit extracts on clinical strains of Candida albicans

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Publicado: sept. 30, 2024
DOI: https://doi.org/10.21676/2389783X.6122
Palabras clave:
Crescentia cujete, Antifungal Agents, Plant Extracts, Candida albicans, Drug Resistance, Fungal

Contenido principal del artículo

Walter Ángel Martínez-De La Rosa
Universidad Metropolitana
Xilene Mendoza-Sánchez
Universidad Metropolitana
Liliana M Ruiz-Tafur
Universidad Metropolitana
Josefina Guzmán-Acuña
Universidad Metropolitana
Yoleydis Elena Collazos-Lara
Universidad Metropolitana
Osmar Pérez-Pérez
Universidad Metropolitana
Alexander Rodríguez-Sanjuán
Universidad del Norte
Yina P. García-Toscano
Universidad Metropolitana
Alfonso Bettin-Martínez
Universidad Metropolitana

Resumen

Candidiasis is a common fungal infection globally, ranging from superficial to severe invasive forms that can be life-threatening. This study aimed to evaluate the antifungal effects of ethanol and n-hexane extracts from Crescentia cujete fruit against Candida albicans strains. Crescentia cujete fruit pulp was dried and extracted using Soxhlet with ethanol and n-hexane. Two reference strains of C. albicans (ATCC 10231 and 90028) and 12 clinical isolates from vaginal smears were tested under various conditions: without extract, with serial dilutions of ethanol or n-hexane extracts, and with ethanol and n-hexane at the same concentrations. Growth was measured using MicroELISA equipment. Results showed that n-hexane and ethanol extracts significantly reduced the growth of 9/12 and 4/12 clinical strains, respectively, and also affected the ATCC 90028 and ATCC 10231 strains. A direct correlation was observed between growth inhibition and extract concentration, with n-hexane exhibiting a stronger correlation (Spearman's Rho=0.609, p<0.05) compared to ethanol (Spearman's Rho=0.387, p<0.05). This study is the first to demonstrate the antifungal effects of Crescentia cujete fruit extracts on clinical Candida albicans strains, suggesting that a low-polarity compound in the fruit inhibits Candida albicans, including the ATCC 1031 strain resistant to common antifungals.

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Cómo citar
Martínez-De La Rosa, W. Ángel, Mendoza-Sánchez, X., Ruiz-Tafur, L. M., Guzmán-Acuña, J., Collazos-Lara, Y. E., Pérez-Pérez, O., … Bettin-Martínez, A. (2024). Antifungal effect of Crescentia cujete fruit extracts on clinical strains of Candida albicans. Duazary, 21(3). https://doi.org/10.21676/2389783X.6122
Número
Vol. 21 Núm. 3 (2024): (julio-septiembre)
Sección
Artículo de investigación científica y tecnológica
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