Evaluation of non-lethal procedures for gonadal analysis of the West Indian topshell, Cittarium pica (Gastropoda: Trochida)
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To enable the regular and harmless monitoring of the gonadal development of the threatened tropical marine gastropod Cittarium pica, non-lethal procedures for gonadal analysis of adult individuals were evaluated. Ingestion and survival rates were examined in experimental groups of snails that were untreated (controls) and treated with procedures such as shell drilling (D); shell drilling and gonadal biopsy (D+B); shell drilling, gonadal biopsy, and covering the hole in the shell with a soft cover made with soft commercial modeling clay (D+B+SC); shell drilling, gonadal biopsy, and covering the hole of the shell with a hard cover made with acrylic resin (D+B+HC). The different procedures applied did not affect the ingestion rate of the snails, except for those individuals exposed to the D+B+SC treatment, which exhibited a decrease ingestion rate compared to the controls. All treatments caused a decrease in the survival rates of the snails compared to the controls, with shell drilling responsible for a 45 % decrease, and the gonadal biopsy for an additional 39 %. Covering the hole in the shell did not improve these values. The results indicate a high sensitivity of C. pica to the manipulation of its shell and tissues, ruling out the use of these procedures for this threatened species.
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