Evaluation of non-lethal procedures for gonadal analysis of the West Indian topshell, Cittarium pica (Gastropoda: Trochida)

Contenido principal del artículo

Judith Barros
Christian Erazo
Luz A. Velasco

Resumen

To enable the regular and harmless monitoring of the gonadal development of the threatened tropical marine gastropod Cittarium pica, non-lethal procedures for gonadal analysis of adult individuals were evaluated. Ingestion and survival rates were examined in experimental groups of snails that were untreated (controls) and treated with procedures such as shell drilling (D); shell drilling and gonadal biopsy (D+B); shell drilling, gonadal biopsy, and covering the hole in the shell with a soft cover made with soft commercial modeling clay (D+B+SC); shell drilling, gonadal biopsy, and covering the hole of the shell with a hard cover made with acrylic resin (D+B+HC). The different procedures applied did not affect the ingestion rate of the snails, except for those individuals exposed to the D+B+SC treatment, which exhibited a decrease ingestion rate compared to the controls. All treatments caused a decrease in the survival rates of the snails compared to the controls, with shell drilling responsible for a 45 % decrease, and the gonadal biopsy for an additional 39 %. Covering the hole in the shell did not improve these values. The results indicate a high sensitivity of C. pica to the manipulation of its shell and tissues, ruling out the use of these procedures for this threatened species.

Descargas

Los datos de descargas todavía no están disponibles.

Detalles del artículo

Cómo citar
Barros, J., Erazo , C., & Velasco , L. A. (2025). Evaluation of non-lethal procedures for gonadal analysis of the West Indian topshell, Cittarium pica (Gastropoda: Trochida). Intropica. https://doi.org/10.21676/23897864.6132
Sección
Artículo de investigación científica y tecnológica

Citas

Acosta-Salmón, H. and Southgate, P.C. (2004). Use of a biopsy technique to obtain gonad tissue from the blacklip pearl oyster Pinctada margaritifera (L.). Aquaculture Research, 35, 93-96. https://doi.org/10.1111/j.1365-2109.2004.00988.x

Beaver, C.E., Geda, S.R., and Johnson, N.A. (2019). Standardizing a non-lethal method for characterizing the reproductive status and larval development of freshwater mussels (Bivalvia: Unionida). Journal of Visualized Experiments, 152, e60244. https://doi.org/10.3791/60244

Bell, L. (1992). Reproduction and larval development of the West Indian topshell, Cittarium pica (Trochidae) in the Bahamas. Bulletin Marine Science, 51(2), 250-266.

Berzins, I.K., and Smolowitz, R. (2006). Diagnostic Techniques and Sample Handling. In: G.A. Lewbart (Ed.). Invertebrate Medicine (pp 263-274), Blackwell Publishing Ltd. Oxford.

Carreño, A., Velasco, L.A., and Toro, B. (2021). Effect of diet and water quality on the energetic physiology of the West Indian top shell Cittarium pica. Aquaculture, 531, 735889. https://doi.org/10.1016/j.aquaculture.2020.735889

Chasqui, V.L., Alvarado-Chacón, E.M., Ardila, N., Borrero-Pérez, G.H., Campos N.H., and Mejía-Quintero, K. (Eds.). (2022). Libro rojo de invertebrados marinos de Colombia. Instituto de Investigaciones Marinas y Costeras INVEMAR, Ministerio de Ambiente y Desarrollo Sostenible. Serie de publicaciones generales de INVEMAR No. 122. Santa Marta, Colombia. 388. ISBN digital: 978-958-8935-76-8.

Correa, T., Toro, B., and Rosique, J. (2012). Some aspects of the bioecology of the west indian topshell Cittarium pica (Mollusca : Gastropoda) in the Darien Colombian Caribbean. Boletín Científico Museo de Historia Natural, 16, 162-172.

Daza-Guerra, C.A., Martínez-Hernández, N.J., and Narváez-Barandica, J.C. (2018). Population aspects of burgao Cittarium pica (Gastropoda: Tegulidae) in the rocky shore of Santa Marta, Magdalena, Colombia. Revista Mexicana de Biodiversidad, 89, 430-442. https://doi.org/10.22201/ib.20078706e.2018.1.2356

Debrot, A. (1990). Survival, growth and fecundity of the west indian topshell, Cittarium pica (Linnaeus), in various rocky intertidal habitats of the Exuma Cays, Bahamas. The Veliger, 33, 363-371.

Dobson, G., and Lee, C.L. (1996). Improved method of determining the sex of the marine topshell, Trochus niloticus (Mollusca: Gastropoda) for spawning. Aquaculture, 139, 329-331.

Encena, V.C., De La Pena, M., and Balinas, V.T. (2013). Evaluation of density and cage design for the nursery and grow-out of the tropical abalone Haliotis asinina Linne 1758. Journal of Shellfish Research, 32(1), 21–27.

Guallart, J., Ferranti, M.P., Bacigalupo, L., and Chiantore, M. (2020). In vivo magnetic resonance imaging to assess the sexual maturity of the endangered limpet Patella ferruginea. Journal of Molluscan Studies, 86, 422–426. https://doi.org/10.1093/mollus/eyaa021

Guete-Salazar, C., Barros, J., and Velasco, L.A. (2021). Settlement and culture of post-larvae, spat and juveniles of the West Indian topshell, Cittarium pica (Linne, 1758). Aquaculture, 543, 737029. https://doi.org/10.1016/j.aquaculture.2021.737029

Khor, S., Wood, S.A., Salvitti, L., Ragg, N.L.C., Taylor, D., McNabb, P., and Cary, S.C. (2013). Development of a non-lethal biopsy technique for estimating total tetrodotoxin concentrations in the grey side-gilled sea slug Pleurobranchaea maculata. Toxicon, 74, 27–33. https://doi.org/10.1016/j.toxicon.2013.07.012

Lee, J.S., Ju, S.M., Park. J.S., Park, Y.G., Shin, Y.K., and Park, J.J. (2010). Germ cell aspiration (GCA) method as a non-fatal technique for sex identification in two bivalves (Gomphina veneriformis and Tegillaca granosa). Development and Reproduction, 14(1), 7-11.

Pereira, L., and Rasse, S. (2007). Evaluation of growth and survival of juveniles of the Japanese abalone Haliotis discus hannai in two culture systems suspended in tanks. Journal of Shellfish Research, 26(3), 769–776. https://doi.org/10.2983/0730-8000(2007)26[769:EOGASO]2.0.CO;2

Osorno, A., and Diaz, J.M. (2006). Explotación, usos y estado actual de la cigua o burgao Cittarium pica (Mollusca: Gastropoda: Trochidae) en la costa continental del Caribe colombiano. Boletín de Investigaciones Marinas y Costeras, 35, 133-148.

Osorno, A., Gil, D.L. and Gómez, L.A. (2009). Plan de investigación para la conservación de Cittarium pica (L. 1758). Invemar, Serie de publicaciones especiales 16.

Ramirez-Santana, B.P., Alvarez-Garcia, I.L., Avila-Poveda, O.H., Arellano-Martinez, M., and Ospina-Garces, S.M. (2024). Seasonal dimorphism as an expression of sexual dimorphism: Influence of gonad maturity on the body shape of a rocky intertidal polyplacophoran. Zoology, 167: e126224. https://doi.org/10.1016/j.zool.2024.126224

Randall, H.E. (1964). A study of the growth and other aspects of the biology of the West Indian Top Shell Cittarium pica (Linnaeus). Bulletin Marine Science, 14, 424-443.

Robertson, R. (2003). The edible West Indian Whelk Cittarium pica (Gastropoda: Trochidae): natural history with new observations. Proceedings of the Academy of Natural Sciences of Philadelphia, 153, 27-47.

Saha, S., and Layzer, J.B. (2008). Evaluation of a nonlethal technique for determining sex of freshwater mussels. Journal of the North American Benthological Society, 27(1), 84-89.

Seinor, K. and Benkendorff, K. (2023). Shell drilling and gonad biopsies provide a rapid, nonlethal method for in situ assessment of reproductive periodicity in a turbinid gastropod. Journal of Molluscan Studies, 89, eyad012. https://doi.org/10.1093/mollus/eyad012.

Silva-Aciares, F.R., Carvajal, P.O., Mejías, C.A., and Riquelme, C.E. (2011). Use of macroalgae supplemented with probiotics in the Haliotis rufescens (Swainson, 1822) culture in Northern Chile. Aquaculture Research, 42, 953-961.

Svärdh, L. (2003). Tissue sampling from live blue mussels, Mytilus edulis, a field study from the Swedish west coast. Journal of Sea Research, 49, 221-225.

Tzakiris, E.T., Randklev, C.R., and Conway, K.W. (2016). Effectiveness of a nonlethal method to quantify gamete production in freshwater mussels. Freshwater Science, 35(3), 958–973.

Velasco, L.A., and Barros, J. (2017). Spawning and early development of the West Indian top shell, Cittarium pica (Linnaeus, 1758), under ex-situ conditions. Aquatic Living Resources, 30, 22. https://doi.org/10.1051/alr/2017022

Velasco, L.A., and Barros, J. (2018). Experimental culture of larvae, post-larvae and juveniles of the West Indian top shell, Cittarium pica (Linnaeus, 1758). Aquaculture Research, 49, 1628-1637. https://doi.org/10.1111/are.13618

Velasco, L.A., and Barros, J. (2019). Survival and growth of the Caribbean scallops, Argopecten nucleus and Nodipecten nodosus, in suspended systems at different culture depths and net replacement frequencies. Aquaculture Environment Interactions, 11: 537–549. https://doi.org/10.3354/aei00334

Velasco, L.A., Barros, J., Guerrero, A., 2009. Growth and survival of the Caribbean scallops Argopecten nucleus and Nodipecten nodosus in suspended culture at the Taganga Bay, Colombia. Aquaculture Research, 40, 687–695.

Velasco, L.A., Villarruel, Y., and Toro, B. (2019). Nursery culture of wild and hatchery-produced juveniles of the West Indian top shell, Cittarium pica, fed microalgal and artificial biofilms. Aquaculture International, 27, 1289–1299. https://doi.org/10.1007/s10499-019-00386-2

Yang, H., Supan, J., Guo, X., and Tiersch, T.R. (2013). Nonlethal sperm collection and cryopreservation in the Eastern Oyster Crassostrea virginica. Journal Shellfish Research, 32, 429-437. https://doi.org/10.2983/035.032.0217

Yusup, D.S., Mahardika, I.G., Suarna, I.W., and Giri, I.N.A. (2020). Feeding preference and growth response of early adults abalone, Haliotis squamata on some macroalgae. Biodiversitas, 21(9), 4369-4375. https://doi.org/10.13057/biodiv/d210950

Zar, J.H., (1999). Biostatistical analysis. Pearson Education India.

Artículos más leídos del mismo autor/a

Artículos similares

1 2 > >> 

También puede {advancedSearchLink} para este artículo.